Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: Expression of PDIA3 in various TCGA tumor tissues (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: Protein expression levels of PDIA3 in various HPA tumor tissues.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: (a) Expression of PDIA3 gene in normal tissue and tumor tissue. (b) Relationship between PDIA3 expression and tumor staging.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: Expression of PDIA3 in cervical cancer and adjacent cancer tissues (IHC 200×). (a) and (b) The positive expression of PDIA3 protein in cervical cancer samples. (c) and (d) The negative expression of PDIA3 protein in normal tissue samples.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: Expression of PDIA3 protein in cervical cancer tissues and adjacent cancer tissues [ n (%)].

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: Relationship between PDIA3 protein expression and clinicopathological characteristics of cervical cancer [ n (%)].

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: (a) Relationship between PDIA3 expression and overall survival (OS). (b) Relationship between PDIA3 expression and disease-specific survival (DSS). (c) Relationship between PDIA3 expression and disease-free interval (DFI). (d) Relationship between PDIA3 expression and progression-free interval (PFI).

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: Correlation between PDIA3 expression and DNA methylation.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing, DNA Methylation Assay

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: (a) Relationship between the PDIA3 expression and B cell memory in cervical cancer. (b) Relationship between the PDIA3 expression and T cell regulatory in cervical cancer. (c) Relationship between the PDIA3 expression and monocytes in cervical cancer. (d) Relationship between the PDIA3 expression and macrophages M2 in cervical cancer. (e) Relationship between the PDIA3 expression and NK cell activated in cervical cancer. (f) Relationship between the PDIA3 expression and mast cells activated in cervical cancer.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: (a) Construction of PPI protein interaction network of PDIA3 gene. (b)–(k) Correlation between related genes and PDIA3 expression [(b): PPIB. (c): HSP90B1. (d): P4HB. (e): PDIA4. (f): HYOU1. (g): PDIA6. (h): MANF. (i): CRELD2. (j): HSPA5. (k): MYDGF (C19ORF10)]. (l) The expression of 10 target genes in each tumor.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: (a) PDIA3 differentially co-expressed top 50 positive related genes in cervical cancer. (b) PDIA3 differentially co-expressed top 50 negative related genes in cervical cancer.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques:

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: GO analysis of PDIA3 differentially co-expressed genes.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques: Glycoproteomics, Membrane, Activity Assay, Protein Binding, RNA Binding Assay, Sequencing, Binding Assay, Transferring

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: KEGG bubble plot of PDIA3 differentially co-expressed top 50 genes.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques:

Journal: International Journal of Genomics

Article Title: Expression and Prognostic Significance of PDIA3 in Cervical Cancer

doi: 10.1155/2022/4382645

Figure Lengend Snippet: KEGG pathway of PDIA3 differentially co-expressed genes.

Article Snippet: The main materials include antigen repair solution EnVisionTM FLEX+, mouse, and high pH (link; Agilent Technologies, Glostrup, Denmark); anti body diluent with background reducing components (Agilent Technologies); endogenous biotin blocking kit (Maixin Biotechnology, Fuzhou, China); ultrasensitive SP kit (Maixin Biotechnology); rat IgG-immuno-histochemical kit (Boster Biological Technology, Wuhan, China); PDIA3 primary antibody (Boster Biological Technology); and cervical cancer tissue microarray HUteS136Su01 (Shanghai Outdo Biotech Company, Shanghai, China).

Techniques:

Journal: Aging Cell

Article Title: The p53/miRNAs/Ccna2 pathway serves as a novel regulator of cellular senescence: Complement of the canonical p53/p21 pathway

doi: 10.1111/acel.12918

Figure Lengend Snippet: miR‐124, miR‐34a, and miR‐29a/b/c were significantly up‐regulated during Aging process. (a) Representative photographs of 2‐month‐old male C57BL/6 mouse and 20‐month‐old male C57BL/6 mouse. (b) The expression p16 levels in the kidney from 2‐month‐old male C57BL/6 mouse and 20‐month‐old male C57BL/6 mouse. (c) The most differentially expressed miRNAs in the kidney from 2‐month‐old male C57BL/6 mouse and 20‐month‐old male C57BL/6 mouse using miRNA microarray ( n = 3). The color is determined by the ratio between the miRNA signal value of 20‐month and 2‐month mouse kidney. (d–h) The expression levels of miRNAs in the kidney, heart, brain, lung, and liver tissues from Aging mouse and Young mouse. Columns, mean of three independent experiments ( n = 3); bars, SEM . * p < 0.05; ** p < 0.01, compared to the Young mouse tissue. (i) The miRNA expression levels in P3 MEF and P9 MEF. Columns, mean of four independent experiments ( n = 4); bars, SEM . * p < 0.05, comparison between two groups as indicated

Article Snippet: The transferred PVDF membrane was then incubated with rabbit anti‐p16 polyclonal antibody (10883‐1‐AP, Protein Tech, China), mouse anti‐CCNA2 monoclonal antibody (ab38, Abcam, USA), anti‐p53 polyclonal antibody (10442‐1‐AP, Protein Tech, China), or anti‐p21 polyclonal antibody (sc‐471, Santa Cruz Biotechnology, USA), followed by the respective secondary antibodies conjugated with horseradish peroxidase (HR) and subjected to a commercial enhanced chemiluminescence (ECL) kit (Pierce, USA).

Techniques: Expressing, Microarray, Comparison

Journal: Aging Cell

Article Title: The p53/miRNAs/Ccna2 pathway serves as a novel regulator of cellular senescence: Complement of the canonical p53/p21 pathway

doi: 10.1111/acel.12918

Figure Lengend Snippet: miR‐124 significantly promoted the replicative senescence of MEF cells. (a) Representative photographs of SA‐β‐gal staining of MEF cells transfected with Agomir NC, Agomir 124, Agomir 34a, and Agomir 29a. (b) The average SA‐β‐gal staining MEF cells transfected with Agomir NC, Agomir 124, Agomir 34a, and Agomir 29a. * p < 0.05; ** p < 0.01, compared to the Agomir NC‐transfected MEFs. (c) The p16 expression in Agomir NC, Agomir 124, Agomir 34a, and Agomir 29a‐transfected MEFs. (d) Effect of miR‐124, miR‐34a, and miR‐29a on cell viability of MEFs transfected with Antagomir NC, Agomir 124, Agomir 34a, and Agomir 29a. (e) Representative images of cells stained with DAPI (blue fluorescence) and BrdU, as a measurement of DNA synthesis (green fluorescence) in Agomir NC, Agomir 124, Agomir 34a, and Agomir 29a‐transfected MEF cells. (f) Representative photographs of SA‐β‐gal staining of MEF cells transfected with miRNA inhibitors, including Antagomir NC, Antagomir 124, Antagomir 34a, Antagomir 29a, and all three inhibitors (MIX). (g) The average SA‐β‐gal staining MEF cells transfected with Antagomir NC, Antagomir 124, Antagomir 34a, Antagomir 29a, and MIX. * p < 0.05; ** p < 0.01, compared to the Antagomir NC‐transfected MEFs. (h) The p16 expression in miRNA inhibitor‐transfected MEFs

Article Snippet: The transferred PVDF membrane was then incubated with rabbit anti‐p16 polyclonal antibody (10883‐1‐AP, Protein Tech, China), mouse anti‐CCNA2 monoclonal antibody (ab38, Abcam, USA), anti‐p53 polyclonal antibody (10442‐1‐AP, Protein Tech, China), or anti‐p21 polyclonal antibody (sc‐471, Santa Cruz Biotechnology, USA), followed by the respective secondary antibodies conjugated with horseradish peroxidase (HR) and subjected to a commercial enhanced chemiluminescence (ECL) kit (Pierce, USA).

Techniques: Staining, Transfection, Expressing, Fluorescence, DNA Synthesis

Journal: Aging Cell

Article Title: The p53/miRNAs/Ccna2 pathway serves as a novel regulator of cellular senescence: Complement of the canonical p53/p21 pathway

doi: 10.1111/acel.12918

Figure Lengend Snippet: Ccna2 significantly delayed the cellular senescence. (a) The Ccna2 mRNA and protein levels in MEFs transfect with siNC and siCcna2‐1/2/3. (b) Representative images of cells stained with DAPI (blue fluorescence) and BrdU (green fluorescence) in siNC‐ and siCcna2‐1/2/3‐transfected MEF cells. (c) Effect of siCcna2 on cell viability of MEFs transfected with siNC and siCcna2‐1/2/3. (d) Representative photographs of SA‐β‐gal staining of MEF cells transfected with siNC and siCcna2‐1/2/3. (e). The average SA‐β‐gal staining MEF cells transfected with siNC and siCcna2‐1/2/3. ** p < 0.01, compared with the blank and siNC‐transfected MEFs. (f) The p16 expression in siNC‐ and siCcna2‐1/2/3‐transfected MEFs (left) or NIH/3T3 cells (right). (g) Effect of Ccna2 overexpression on cell viability of MEFs infected with Lenti‐NC and Lenti‐Ccna2. (h) The p16 and Ccna2 protein expression in Lenti‐NC‐ and Lenti‐Ccna2‐infected MEFs. (i) Representative photographs of SA‐β‐gal staining of MEF cells infected with Lenti‐NC and Lenti‐Ccna2. (j) The average SA‐β‐gal staining MEF cells infected with Lenti‐NC and Lenti‐Ccna2. ** p < 0.01, compared to the Lenti‐NC‐infected MEFs

Article Snippet: The transferred PVDF membrane was then incubated with rabbit anti‐p16 polyclonal antibody (10883‐1‐AP, Protein Tech, China), mouse anti‐CCNA2 monoclonal antibody (ab38, Abcam, USA), anti‐p53 polyclonal antibody (10442‐1‐AP, Protein Tech, China), or anti‐p21 polyclonal antibody (sc‐471, Santa Cruz Biotechnology, USA), followed by the respective secondary antibodies conjugated with horseradish peroxidase (HR) and subjected to a commercial enhanced chemiluminescence (ECL) kit (Pierce, USA).

Techniques: Staining, Fluorescence, Transfection, Expressing, Over Expression, Infection

Journal: Aging Cell

Article Title: The p53/miRNAs/Ccna2 pathway serves as a novel regulator of cellular senescence: Complement of the canonical p53/p21 pathway

doi: 10.1111/acel.12918

Figure Lengend Snippet: Ccna2 was the common target of miR‐124 and miR‐29. (a) Alignment and schematic diagram of Ccna2 3'UTR and the predicted miRNA binding sites. (b) Determination of luciferase activity. Cells were co‐transfected with Ccna2‐3'UTR with Agomir NC, Agomir 124, Agomir 34a, or Agomir 29a. Columns, mean of three independent experiments done in duplicate ( n = 3); bars, SEM . ** p < 0.01, compared with Agomir NC‐transfected cells. (c) Cells were co‐transfected with Mut‐3'UTR‐124 with Agomir NC or Agomir 124, or co‐transfected with Mut‐3'UTR‐29 with Agomir NC or Agomir 29a. Columns, mean of at least three independent experiments done in duplicate; bars, SEM . (d) Real‐time qPCR was used to monitor the Ccna2 expressions in MEF cells transfected with Agomir NC, Agomir 124, Agomir 34a, and Agomir 29a. * p < 0.05, comparison between two groups as indicated. (e) The Ccna2 protein expression levels in MEF cells transfected with Agomir NC, Agomir 124, Agomir 34a, and Agomir 29a. (f and g). Ccna2 protein expression levels in Aging mouse (f) and senescent MEF cells (g). (h and i) Representative photographs (h) and average (i) of SA‐β‐gal staining of MEF cells infected with Lenti‐NC and Lenti‐Ccna2 after transfected with Agomir 124, Agomir 29a, or Agomir NC. * p < 0.05, ** p < 0.01, comparison between two groups as indicated. (j and k) The p16 protein expression and the intensity ratio between p16 and GAPDH in MEF cells infected with Lenti‐NC and Lenti‐Ccna2 after transfected with Agomir 124, Agomir 29a, or Agomir NC. * p < 0.05, comparison between two groups as indicated

Article Snippet: The transferred PVDF membrane was then incubated with rabbit anti‐p16 polyclonal antibody (10883‐1‐AP, Protein Tech, China), mouse anti‐CCNA2 monoclonal antibody (ab38, Abcam, USA), anti‐p53 polyclonal antibody (10442‐1‐AP, Protein Tech, China), or anti‐p21 polyclonal antibody (sc‐471, Santa Cruz Biotechnology, USA), followed by the respective secondary antibodies conjugated with horseradish peroxidase (HR) and subjected to a commercial enhanced chemiluminescence (ECL) kit (Pierce, USA).

Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Comparison, Expressing, Staining, Infection

Journal: Aging Cell

Article Title: The p53/miRNAs/Ccna2 pathway serves as a novel regulator of cellular senescence: Complement of the canonical p53/p21 pathway

doi: 10.1111/acel.12918

Figure Lengend Snippet: p53/p53 responsive miRNA/Ccna2 pathway served as a novel regulator of cellular senescence independent of p53/p21 pathway. (a) The p21 mRNA and protein levels in MEFs transfect with siNC, sip21‐1/2/3. (b) Representative photographs of SA‐β‐gal staining of MEF cells transfected with siNC and sip21 (sip21‐3). (c) The average SA‐β‐gal staining MEF cells transfected with siNC and sip21. *** p < 0.001, compared to the siNC‐transfected MEFs. (d) The miRNA expression levels in sip21‐transfected MEF cells. Columns, mean of four independent experiments ( n = 3); bars, SEM . * p < 0.05; ** p < 0.01, comparison between two groups as indicated. (e) Representative photographs of SA‐β‐gal staining of MEF cells co‐transfected with siNC or sip21, and Agomir 124/Agomir 29a or Agomir NC. (f) The average SA‐β‐gal staining MEF cells transfected with siNC or sip21, and Agomir 124/Agomir 29a or Agomir NC. * p < 0.05, compared to the siNC and Agomir 124/Agomir 29a‐transfected MEFs. (g) The p16 and p21 protein expression of MEF cells co‐transfected with siNC or sip21, and Agomir 124/Agomir 29a or Agomir NC were evaluated by western blot. (h) The p16, p21, and CCNA2 protein expression of MEF cells co‐transfected with siNC or sip21, and siNC or siCcna2 (siCcna2‐2). (i) Representative photographs of SA‐β‐gal staining of MEF cells co‐transfected with siNC or sip21, and siNC or siCcna2. (j) The average SA‐β‐gal staining MEF cells transfected with siNC or sip21, and siNC or siCcna2. ** p < 0.01, compared to the siNC‐ and siCcna2‐transfected MEFs

Article Snippet: The transferred PVDF membrane was then incubated with rabbit anti‐p16 polyclonal antibody (10883‐1‐AP, Protein Tech, China), mouse anti‐CCNA2 monoclonal antibody (ab38, Abcam, USA), anti‐p53 polyclonal antibody (10442‐1‐AP, Protein Tech, China), or anti‐p21 polyclonal antibody (sc‐471, Santa Cruz Biotechnology, USA), followed by the respective secondary antibodies conjugated with horseradish peroxidase (HR) and subjected to a commercial enhanced chemiluminescence (ECL) kit (Pierce, USA).

Techniques: Staining, Transfection, Expressing, Comparison, Western Blot